Tender for Dna Extraction Kit for Molecular Biology, Qty: 6 5...

Purchaser's Detail

Purchaser : INDIAN COUNCIL OF FORESTRY RESEARCH AND EDUCATION
Ministry Of Environment Forest And Climate Change
NA
Vinutha C 560003, Institute of Wood Science and Technology 18th cross malleswaram 2 15 NaCl, For Molecular Biology
Country: India
Email: buycon1.icfrei.ka@gembuyer.in
Website: www.gem.gov.in

Tender's Details

BOQ Item Description: For Molecular Biology, this kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 200 mg of wet weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with Super Plant Extraction Buffer. Super Plant Extraction Buffer contains CTAB Cetyltrimethylammonium bromide, a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform isoamyl alcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column Capped that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.
BOQ Title: Purchase of Chemicals
Item Title: Dna Extraction Kit for Molecular Biology
Item Quantity: 6
Unit of Measure: 50preps
Delivery Period (In number of days): 15
Start Date: 15-02-2024 12:19 PM
End Date: 26-02-2024 12:00 PM